Device

Part:BBa_K190039:Design

Designed by: Nienke Kuipers   Group: iGEM09_Groningen   (2009-09-08)


Arsenic Accumulation device (Low constitutive promoter + fMT + GlpF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 81
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 170
    Illegal NgoMIV site found at 554
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The two genes were cloned in such a way that a polycistronic mRNA will be formed, but after translation two separate proteins will be produced as both still contain a stop-codon.


Source

The device is composed from BBa_K190031, which contains the low constitutive promoter (BBa_J23109) together with fMT (BBa_K190019) , this is fused with BBa_K190028, GlpF the arsenic transporter.

References